During PCR amplification, it can be difficult to determine if it is a single PCR product. TA cloning is often used to determine the respective fragments. Due to the reaction of PCR applicable polymerase having terminal transfer activity, it usually adds A in the 3′ end of the PCR product. The PCR product can be connected using a T-vector.

Workflow:

Figure 6. Workflow of TA Cloning

Sample requirements:

Turnaround Time:

The standard turnaround time using the workflow (above) is approximately 5 working days.

Completion Milestone and Results Submission:

Completion milestone:
Project completion is based on a quality score of Q 20.
Result submission:
Results include the sequencing peak figure, basic analysis, and a final report.